PDLIM1 Polyclonal Antibody

Catalogue Numbers: BS62275-50, BS62275-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O00151

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB IHC IP IF

All Applications: WB:1:500~1:1000 IHC:1:50~1:200 IP:1:50~1:200 IF:1:50~1:200

Background: CLP36, also known as PDLIM1, CLIM1 or Elfin, is a 329 amino acid cytoplasmic protein that associates with Actin stress fibers at the cytoskeleton. Expressed at high levels in skeletal muscle and heart and at lower levels in colon, small intestine, spleen, lung, placenta, kidney, liver, thymus and pancreas, CLP36 functions as a cytoskeletal protein that is thought to act as an adaptor, bringing target proteins to the cytoskeleton. Specifically, CLP36 interacts with Clik1 (a kinase) and recruits Clik1 to α-actinin-1, thereby facilitating the association of Clik1 with Actin stress fibers. CLP36 contains one PDZ domain and one LIM zinc-binding domain through which it conveys its protein-protein binding capabilities. Human CLP36 shares 88% sequence similarity with its rat counterpart, suggesting a conserved function between species.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PDLIM1 polyclonal antibody detects endogenous levels of PDLIM1 protein.

Extra Notes: Western blot (WB) analysis of PDLIM1 polyclonal antibody at 1:500 dilution
LaneA:HEK293T whole cell lysate
LaneB:Hela whole cell lysate
LaneC:H1688 whole cell lysate
LaneD:The Heart tissue lysate of Mouse
LaneE:The Muscle tissue lysate of Mouse
LaneF:The Heart tissue lysate of Rat
LaneG:The Muscle tissue lysate of Rat

Bioworld Molecular Weight: ~ 36 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: PDZ and LIM domain protein 1; C-terminal LIM domain protein 1; Elfin; LIM domain protein CLP-36; PDLIM1; CLIM1; CLP36

Immunogen: Synthetic peptide, corresponding to Human PDLIM1.

Conjugate: Unconjugated

Modification: Unmodified