c-Met (E999) Polyclonal Antibody

Catalogue Numbers: BS1793-50, BS1793-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P08581

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB IHC IF

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Background: c-Met, a member of the tyrosine kinase superfamily, is the receptor for hepatocyte growth factor, also known as scatter factor (HGF/SF). The mature c-Met protein is a disulfide-linked heterodimer with Mr=190 kDa composed of a heavily glycosylated alpha subunit that is completely extracellular in localization, and a beta subunit comprised of an extracellular ligand binding domain, a single transmembrane domain, and a cytoplasmic tyrosine kinase domain. Cells expressing c-Met include epithelial cells, endothelial cells, blood cells of various types, and glomerular mesenchymal cells.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: c-Met (E999) polyclonal antibody detects endogenous levels of c-Met protein.

Extra Notes: Western blot (WB) analysis of c-Met (E999) pAb at 1:500 dilution
Lane1:HEK293T whole cell lysate(40ug)
Lane2:A375 whole cell lysate(40ug)
Lane3:SGC7901 whole cell lysate(40ug)
Lane4:SK-OVCAR3 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 156 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Hepatocyte growth factor receptor; HGF receptor; HGF/SF receptor; Proto-oncogene c-Met; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met; MET

Immunogen: Synthetic peptide, corresponding to amino acids 961-1010 of Human c-Met.

Conjugate: Unconjugated

Modification: Unmodified