MDFI (P134) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS3882-50, BS3882-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q99750

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF

All Applications: WB: 1:500~1:1000
IF 1:50~1:200

Background: MDFI is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures. MDFI an also interfere with the DNA-binding activity of Myod family members and plays an important role in trophoblast and chondrogenic differentiation.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MDFI (P134) polyclonal antibody detects endogenous levels of MDFI protein.

Bioworld Molecular Weight: ~ 25 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of MDFI (P134) pAb at 1:500 dilution
Lane 1: SGC7901 whole cell lysate (40ug)
Lane 2: PC3 whole cell lysate (40ug)
Lane 3: HepG2 whole cell lysate (40ug)
Lane 4: HCT116 whole cell lysate (40ug)
Lane 5: C6 whole cell lysate (40ug)
Lane 6: 3T3-L1 whole cell lysate (40ug), Immunofluorescence analysis of PC3 cells using MDFI (P134) pAb at dilution of 1:200.

Alternative Name: MyoD family inhibitor; Myogenic repressor I-mf; MDFI

Immunogen: Synthetic peptide, corresponding to amino acids 100-146 of Human MDFI.

Conjugate: Unconjugated

Modification: Unmodified