PBK (I4) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1571-50, BS1571-100

Sizes: 50µl, 100µl

Swiss-Prot: Q96KB5

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: PBK (PDZ binding kinase) belongs to the serine/threonine protein kinase family. It is related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family and phosphorylates MAP kinase p38. It is active only in mitosis. When phosphorylated it forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage. It makes a kinase-substrate complex with cdk1/cyclin B1 and PRC1 on microtubules during mitosis, enhances the cdk1/cyclin B1-dependent phosphorylation of PRC1 and thereby strongly promotes cytokinesis. It may play a role in the activation of lymphoid cells.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: PBK/TOPK (I4) polyclonal antibody detects endogenous levels of PBK/TOPK protein

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of PBK (I4) pAb at 1:500 dilution
Lane 1: PMVEC whole cell lysate (40ug)
Lane 2: K562 whole cell lysate (40ug)
Lane 3: 3T3-L1 whole cell lysate (40ug)
Lane 4: HuT78 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of PBK (I4) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 36 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Lymphokine-activated killer T-cell-originated protein kinase; Cancer/testis antigen 84; CT84; MAPKK-like protein kinase; Nori-3; PDZ-binding kinase; Spermatogenesis-related protein kinase; SPK; T-LAK cell-originated protein kinase; PBK; TOPK

Immunogen: Synthetic peptide, corresponding to the N-terminal of Human PBK.

Conjugate: Unconjugated

Modification: Unmodified