FSHR Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS5724-50, BS5724-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P23945

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC ICC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
ICC: 1:50~1:200

Background: Follicle-stimulating hormone receptor (FSHR) is a 695 amino acid G-protein coupled receptor. FSH binds to the receptor in a hand-clasp fashion via its α and β subunits. While the α subunit of FSH is involved in the binding of FSH to the receptor, the β subunit stabilizes this interaction. Linkage studies suggest that a missense mutation in the FSHR gene can cause reduced FSH binding affinity and lead to a condition known as hypergonadotropic ovarian dysgenesis (ODG). In males however, this mutation does not appear to have a detrimental affect on fertility. It is believed that a mutation in the FSHR gene is also associated with ovarian hyperstimulation syndrome; a condition characterized by the presence of multiple serous and hemorrhagic follicular cysts lined by luteinized cells.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: FSHR polyclonal antibody detects endogenous levels of FSHR protein.

Bioworld Molecular Weight: ~ 78 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of FSHR pAb at 1:500 dilution
Lane 1: A2780 whole cell lysate (20ug)
Lane 2: SK-OVCAR3 whole cell lysate (20ug)
Lane 3: The Testis tissue lysate of Mouse (40ug)
Lane 4: The Ovary tissue lysate of Rat (40ug), Immunocytochemistry stain of A549 cells using FSHR antibody (1:200).

Alternative Name: Follicle-stimulating hormone receptor; FSH-R; Follitropin receptor; LGR1

Immunogen: Synthetic peptide, corresponding to amino acids 330-375 of Human FSHR.

Conjugate: Unconjugated

Modification: Unmodified