IARS2 (Y97) Polyclonal Antibody

Catalogue Numbers: BS2148-50, BS2148-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9NSE4

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:1000~1:2000

Background: The fidelity of protein synthesis requires efficient discrimination of amino acid substrates by aminoacyl-tRNA synthetases. Accurate discrimination of the structurally similar amino acids valine and isoleucine by isoleucyl-tRNA synthetase (IleRS) results, in part, from a hydrolytic editing reaction, which prevents misactivated valine from being stably joined to tRNAIle. IleRS joins Ile to tRNA(Ile) at its synthetic active site and hydrolyzes incorrectly acylated amino acids at its editing active site. A member of the aminoacyl-tRNA synthetase family, human IleRS has been identified as a target of antibodies in the autoimmune disease polymyositis.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IleRS (Y97) polyclonal antibody detects endogenous levels of IleRS protein.

Extra Notes: Western blot (WB) analysis of IleRS (Y97) polyclonal antibody at 1:1000 dilution
Lane1:The Kidney tissue lysate of Mouse(40ug)
Lane2:The Testis tissue lysate of Rat(40ug)
Lane3:HEK293T whole cell lysate(40ug)
Lane4:Panc1 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 114 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Isoleucine--tRNA ligase, mitochondrial; Isoleucyl-tRNA synthetase; IleRS; IleRS

Immunogen: Synthetic peptide, corresponding to amino acids 67-127 of Human IleRS.

Conjugate: Unconjugated

Modification: Unmodified