ZPBP Polyclonal Antibody Sizes: 50µl, 100µl Catalogue Numbers: BS67760-50, BS67760-100 Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Swiss-Prot: Q9BS86 Host: Rabbit Reactivity: Human, Mouse, Rat Applications: WB, IHC, IF/ICC All Applications: WB (1/500 - 1/1000), IHC (1/50 - 1/200), IF/ICC (1/50 - 1/200) Background: ZPBP1 (zona pellucida-binding protein 1) is a 351 amino acid gene product belonging to the zona pellucida-binding protein Sp38 family. ZPBP1 is a secreted protein believed to be involved in gamete interaction during fertilization. ZPBP1 is found on chromosome 7 which is about 158 milllion bases long, encodes over 1,000 genes and makes up about 5% of the human genome. Chromosome 7 has been linked to osteogenesis imperfecta, Pendred syndrome, lissencephaly, citrullinemia and Shwachman-Diamond syndrome. The deletion of a portion of the q arm of chromosome 7 is associated with Williams-Beuren syndrome, a condition characterized by mild mental retardation, an unusual comfort and friendliness with strangers and an elfin appearance. Deletions of portions of the q arm of chromosome 7 are also seen in a number of myeloid disorders including cases of acute myelogenous leukemia and myelodysplasia. Purification and Purity: The antibody was purified by immunogen affinity chromatography. Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity: Recognizes endogenous levels of ZPBP protein. Bioworld Molecular Weight: ~ 34 kDa Note: For research use only, not for use in diagnostic procedure. Extra Notes: Western blot analysis of ZPBP expression in mouse brain (A) whole cell lysates., Immunohistochemical analysis of ZPBP staining in rat ovary formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunofluorescent analysis of ZPBP staining in rat testis cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. Alternative Name: ZPBP1; Zona pellucida-binding protein 1; Sp38 Immunogen: Recombinant protein corresponding to human ZPBP. The exact sequence is proprietary. Conjugate: Unconjugated Modification: Unmodified