Recombinant Human Carbonic Anhydrase IX/CA9 (C-6His)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSH033940-10, PKSH033940-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: Carbonic Anhydrase IX; CA9

Target Symptom: CA9; CA-IX; Carbonic Anhydrase IX; Carbonate dehydratase IX; G250; MN; P54/58N; RCC; RCC-associated protein G250

Research Area: Signal Transduction; Cardiovascular; Cancer; metabolism;

Target Species: Human

Expression Host: HEK293 Cells

Fusion Tag: C-6His

UNIProt ID: Q16790

Accession: Q16790

Background: Carbonic anhydrases IX (CA IX), also known as membrane antigen MN or CA9, is a member of the carbonic anhydrase (CA) family and may be involved in cell proliferation and cellular transformation. CAs are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide (H2O + CO2 = H+ + HCO3–) and thus participate in a variety of biological and physical processes. CA9 is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC. Appears to be a novel specific biomarker for a cervical neoplasia.

Sequence: Gln38-Asp414

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 42 kDa

Observed MW: 45-60 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only