IP3 (Inositol Triphosphate) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK8148-48, ELK8148-96, ELK8148-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: InsP3; Inositol 1,4,5-Trisphosphate; Triphosphoinositol

Assay Type: Competitive Inhibition

Sensitivity: 48.6 pg/mL

Standard: 10000 pg/mL

Detection Range: 156.25-10000 pg/mL

Sample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay Length: 2h

Research Areas: Signal transduction; Metabolic pathway; Endocrinology; Cardiovascular biology; Neuroscience; Hormone metabolism; Bone metabolism;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with IP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to IP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IP3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only