MSH3 (A80) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2419-50, BS2419-100

Sizes: 50µl, 100µl

Swiss-Prot: P20585

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: DNA mismatch repair (MMR) is essential for maintaining the integrity of the genome during replication. This process is highly conserved across bacterial and eukaryotic systems, as many of the genes expressed in bacteria are closely related to the yeast and mammalian homologs. In bacteria two proteins, MutS and MutL, form homodimeric complexes that are responsible for recognizing and facilitating MMR. Human homologs of these proteins include MSH2 and MSH3 (MutS homolog 2 and 3), and the corresponding human homologs of MutL are MLH1, PMS1, PMS2 and MLH3. MSH2 and MSH3 form heterodimers that cooperatively mediate MMR. MLH3 preferentially dimerizes with MLH1 to repair DNA mismatches and restore the stability to the genome.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: MSH3 (A80) polyclonal antibody detects endogenous levels of MSH3 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of MSH3 (A80) pAb at 1:500 dilution
Lane 1: HEK293T whole cell lysate (20ug)
Lane 2: MCF-7 whole cell lysate (20ug)
Lane 3: H9C2 whole cell lysate (20ug)
Lane 4: CT26 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of MSH3 (A80) pAb in paraffin-embedded liver cancer tissue at 1:100.

Bioworld Molecular Weight: ~ 127 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: DNA mismatch repair protein Msh3; hMSH3; Divergent upstream protein; DUP; Mismatch repair protein 1; MRP1; MSH3; DUC1; DUG

Immunogen: Synthetic peptide, corresponding to amino acids 50-100 of Human MSH3.

Conjugate: Unconjugated

Modification: Unmodified