Thrombopoietin Polyclonal Antibody

Catalogue Numbers: BS60467-50, BS60467-100

Sizes: 50µl, 100µl

Alternative Name: Thrombopoietin; C-mpl ligand; ML; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; Myeloproliferative leukemia virus oncogene ligand; THPO; MGDF; TPO; c-Mpl L

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P40225

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Thrombopoietin (TPO or THPO), also known as c-Mpl ligand (c-Mpl L), is a cytokine that plays a central role in megakaryopoiesis by influencing the development and maturation of megakaryocytes and platelet production. TPO is expressed by both mature and progenitor megakaryocytes, as well as by human platelets. Human TPO cDNA encodes a 332 amino acid precursor with a 21 amino acid signal peptide which is cleaved to generate the mature protein. TPO isolated from serum ranges in molecular weight, which suggests that the protein is highly glycosylated. TPO exerts its biological effects through the TPO receptor, c-Mpl. Stimulation of c-Mpl with TPO results in the activation of the Janus tyrosine kinase family members, Tyk 2 and JAK2 which in turn phosphorylate Stat5 and Stat3, causing their nuclear translocation and the transcription of Stat responsive genes.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Thrombopoietin polyclonal antibody detects endogenous levels of Thrombopoietin protein.

Bioworld Molecular Weight: ~ 41 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Thrombopoietin polyclonal antibody at 1:500 dilution
Lane 1: HEK293T cell lysate
Lane 2: Mouse liver tissue lysate
Lane 3: Rat liver tissue lysate

Immunogen: A synthetic peptide corresponding to residues in Human Thrombopoietin.

Conjugate: Unconjugated

Modification: Unmodified