Phospho-CDK1/2 (Thr14) Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB11851-50, MB11851-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: P06493/P24941

Host: Rabbit

Reactivity: Human

Applications: WB, IHC-P, IP,

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20

Background: Cdk2 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. Cdk2 is closely related to cdc2 (cdk1) which has proved useful as a marker of proliferation. Cdk1 and Cdk2 are a catalytic subunits of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 34 kDa; Observed MW: 34 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Cdk1/2 in HepG2, U2OS, U251 lysates using Phospho-CDK1/2 antibody., Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Cdk1/2 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: CDKN2; p33(CDK2)

Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Thr14 of human Cdk1/2

Conjugate: Unconjugated

Modification: Phosphorylated