IDH1 Mouse Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB9026-50, MB9026-100

Product: Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O75874

Host: Mouse

Reactivity: Human, Zebra fish

Applications: WB, IHC

All Applications: WB: 1:1000
IHC: 1:100-1:200

Background: IDH1 is one of three isocitrate dehydrogenases that catalyze the oxidative decarboxylation of isocitrate to α-ketoglutarate (α-KG). These enzymes exist in two distinct subclasses that utilize either NAD or NADP+ respectively, as an electron acceptor. IDH1 is the NADP+-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of IDH1 and does not cross-react with related proteins.

Bioworld Molecular Weight: ~ 46 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of IDH1 Mouse mAb at 1:500 dilution
Lane 1: Hela whole cell lysate
Lane 2: HepG2 whole cell lysate
Lane 3: A431 whole cell lysate
Lane 4: MCF7 whole cell lysate
Lane 5: A549 whole cell lysate
Lane 6: Jurkat whole cell lysate
Lane 5: The kidney tissue lysate of Human
Lane 5: The brain tissue lysate of Human
Lane 5: The liver tissue lysate of Human, Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-IDH1 antibody. Counter stained with hematoxylin.

Alternative Name: Isocitrate dehydrogenase [NADP] cytoplasmic; IDH; Cytosolic NADP-isocitrate dehydrogenase; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase; IDH1; PICD

Immunogen: This antibody is produced by immunizing mice with a recombinant protein corresponding to a region of IDH1.

Conjugate: Unconjugated

Modification: Unmodified