CD4 Rat Monoclonal Antibody

Catalogue Numbers: MB65781-50, MB65781-100

Sizes: 50µl, 100µl

Product: Rat IgG2b. Liquid in PBS, pH 7.3, and 0.02% sodium azide.

Swiss-Prot: P06332

Host: Rat

Reactivity: Mouse

Applications: IF, FC

All Applications: IF (1/50 - 1/200)

Background: Cluster of Differentiation 4 (CD4) is a glycoprotein composed of an amino-terminal extracellular domain (four domains: D1-D4 with Ig-like structures), a transmembrane part, and a short cytoplasmic tail. CD4 is expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells, and plays an important role in the development and activation of T cells. On T cells, CD4 is the co-receptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen–Major Histocompatibility Complex (MHC). Specifically, the D1 domain of CD4 interacts with the β2-domain of the MHC class II molecule. CD4 ensures specificity of the TCR–antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and recruits the tyrosine kinase Lck, which is essential for T cell activation.

Purification and Purity: The monoclonal antibody was affinity-purified from rat antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes mouse CD4

Extra Notes: Flow cytometric analysis of mouse spleen cells using Anti-CD4 Antibody, followed by anti-mouse IgG PE.

Bioworld Molecular Weight: ~ 51 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: T-cell surface glycoprotein CD4; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; CD antigen CD4

Immunogen: Native purified mouse CD4.

Conjugate: Unconjugated

Modification: Unmodified