Human anti-SARS-CoV2 (S-RBD) (Omicron,B.1.1.529) IgG ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK0730-48, ELK0730-96, ELK0730-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: anti-COVID-19; anti-SARS-CoV-2; anti-2019-nCoV

Assay Type: Sandwich

Sensitivity: 0.89 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: serum, plasma and biological fluids

Assay Length: 3.5h

Research Areas: Infectious Diseases

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human anti-SARS-CoV2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human anti-SARS-CoV2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human anti-SARS-CoV2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human anti-SARS-CoV2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only