HSP22 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90666-50, BS90666-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9UJY1(Human) Q9JK92(Mouse) Q9EPX0(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC

All Applications: WB:1:1,000-1:2,000
ICC:1:100-1:500
IHC:1:50-1:200

Background: Crystallins are the major proteins expressed in the vertebrate eye lens, where they maintain the transparency and refractive index of the lens. Crystallins are divided into a, b and g families; b and g-crystallins compose a superfamily. Crystallins usually contain seven distinctive protein regions, including four homologous motifs, a connecting peptide, and N- and C-terminal extensions. a-crystallins consist of three gene products, aA, aB and aC-crystallin, which are members of the small heat shock protein family (HSP20). They are induced by heat shock, and act as molecular chaperones by holding denatured proteins in large soluble aggregates. However, unlike other molecular chaperones, a-crystallins do not renature these proteins. Research indicates that binding occurs between membranes and aC-crystallin. The binding site appears to be at the polar-apolar interface in membrane protein (MIP26) and aC-crystallin; the lipid bilayer becomes less mobile with aC-crystallin binding.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: HSP22 polyclonal antibody detects endogenous levels of HSP22 protein.

Bioworld Molecular Weight: 22 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Hsp22 on different lysates using anti-Hsp22 antibody at 1/1,000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: Mouse skeletal muscle, ICC staining Hsp22 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: HspB8, Alpha crystallin C chain, Alpha-crystallin C chain, Charcot Marie Tooth disease axonal type 2L, Charcot Marie Tooth disease spinal, CMT2L, CRYAC, DHMN 2, DHMN2, E2 induced gene 1 protein, E2-induced gene 1 protein, E2IG1, H11, Heat shock 22kDa protein 8, Heat shock 27kDa protein 8, Heat shock protein 22, Heat shock protein beta 8, Heat shock protein beta-8, Hereditary motor neuropathy distal, HMN 2, HMN2, HMN2A, HSB8, HSPB 8, HspB8, HSPB8, OTTHUMP00000239768, Protein kinase H11, Small stress protein like protein HSP22, Small stress protein-like protein HSP22, Spinal muscular atrophy distal adult autosomal dominant,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified