MPI Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB0150-50, MB0150-100

Product: Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P34949

Host: Mouse

Reactivity: Human, Rat

Applications: WB, ICC

All Applications: WB: 1:500~2000
ICC: 1:200~500

Background: Phosphomannose isomerase catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate and plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib.Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.

Purification and Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of Mannose Phosphate Isomerase and does not cross-react with related proteins

Bioworld Molecular Weight: Predicted band size: 54KDa
Observed band size: 45KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot detection of MPI in Rat kidney, Rat brain, A549 and LNCAP cell lysates and using MPI antibody (1:1000 diluted)., Immunocytochemistry stain of Hela using MPI antibody (1:300).

Alternative Name: Phosphohexomutase; Phosphomannose isomerase; PMI; PMI1; CDG1B

Immunogen: Recombinant full length Human MPI.

Conjugate: Unconjugated

Modification: Unmodified