KLF2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65614-50, BS65614-100

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Swiss-Prot: Q9Y5W3

Host: Rabbit

Reactivity: Human, Mouse, Rat, Pig, Cow, Horse, Rabbit, Sheep,

Applications: ICC

All Applications: ICC=1:100

Background: Kruppel-like factors (KLFs) are a family of broadly expressed zinc finger transcription factors. KLF2 regulates T-cell trafficking by promoting expression of the lipid-binding receptor S1P1 (S1PR1; MIM 601974) and the selectin CD62L (SELL; MIM 153240) (summary by Weinreich et al., 2009 [PubMed 19592277]).[supplied by OMIM, Feb 2011]

Purification and Purity: affinity purified by Protein A

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: KLF2 Polyclonal Antibody detects endogenous levels of KLF2 protein.

Bioworld Molecular Weight: 37kD

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Tissue/cell: Mouse embryos;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15min;Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation:Anti-KLF2 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C; TheSecondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugatedused at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,) was used to stain the cell nuclei

Alternative Name: Kruppel like factor 2; Kruppel like factor; Kruppel like factor LKLF; Kruppel-like factor 2 (lung); Lung krueppel like factor; Lung Kruppel like zinc finger transcription factor; KLF 2; Klf2; KLF2_HUMAN; Krueppel-like factor 2; Lklf; Lung krueppel like factor; Lung krueppel-like factor; Lung Kruppel like zinc finger transcription factor.

Immunogen: KLH conjugated synthetic peptide derived from human KLF2:251-355/355

Conjugate: Unconjugated

Modification: Unmodified