AMACR Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB66173-50, MB66173-100

Product: Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Swiss-Prot: Q9UHK6

Host: Mouse

Reactivity: Human

Applications: WB, IHC

All Applications: WB (1/500 - 1/1000), IHC (1/100 - 1/300)

Background: α-methylacyl-CoA racemase (AMACR), an enzyme localized in peroxisomes and mitochondria, is involved in the β-oxidation of branched-chain fatty acids and fatty acid derivatives . AMACR has been reported to be a biomarker for prostate cancer . The expression of AMACR is also related to other types of cancers such as hepatocellular carcinoma , noninvasive bladder cancer, colorectal cancer and gastric adenocarcinoma.

Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemical analysis of AMACR staining in human prostatic carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6. 0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Molecular Weight: ~ 43 kDa

Specificity: Recognizes endogenous levels of AMACR protein.

Alternative Name: Alpha-methylacyl-CoA racemase; 2-methylacyl-CoA racemase

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human AMACR. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified