NOX4 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90957-50, BS90957-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9NPH5(Human) Q924V1(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC, IHC, IP,

All Applications: WB:1:1,000
ICC:1:50-1:100
IHC:1:50-1:200

Background: The superoxide-generating NADPH oxidase includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with the flavocytochrome, cytochrome b558, to form the active enzyme complex. The p22 and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. Nox4 (also known as Renox) is a renal gp91-phox homolog highly expressed at the site of erythropoietin production in the proximal convoluted tubule epithelial cells of the renal cortex. Nox4 is also expressed in fetal tissues, placenta, glioblastoma and vascular cells. Like gp91-phox, the enzymatic activity of Nox4 produces superoxide anions. In vascular cells, the addition of Angiotensin II increases Nox4 expression, which suggests a role for Nox4 in vascular oxidative stress response. The gene encoding human Nox4 maps to chromosome 11q14.2-q21.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: NOX4 polyclonal antibody detects endogenous levels of NOX4 protein.

Bioworld Molecular Weight: 67 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of NOX4 on PC-12 cell lysates using anti-NOX4 antibody at 1/1,000 dilution., ICC staining NOX4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: KOX-1, NOX4, RENOX, Kidney oxidase-1, Kidney superoxide-producing NADPH oxidase, KOX 1, KOX, Kox-1, NADPH, NADPH oxidase 4, Nox4, NOX4, Renal NAD(P)H-oxidase, RENOX,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified